Product Name: 5-LO (phospho Ser272) Polyclonal Antibody
Catalog No.: ALP0715
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ALOX5
Protein Name: Arachidonate 5-lipoxygenase
Human Gene ID: 240
Human Swiss Prot No.: P09917
Other Name: ALOX5; LOG5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase
Subcellular Location: Cytoplasm . Nucleus matrix . Nucleus membrane ; Peripheral membrane protein . Cytoplasm, perinuclear region . Cytoplasm, cytosol . Nucleus envelope . Nucleus intermembrane space . Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640). .
Expression: Brain,Spleen,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.