Product Name: TLE1/2/3/4 Polyclonal Antibody
Catalog No.: ALT5746
Reactivity: Human;Mouse;Rat
Applications: WB;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name:
Protein Name: TLE1/2/3/4
Human Gene ID: 7088
Human Swiss Prot No.: Q04724/Q04725/Q04726/Q04727
Other Name: similar to transducin-like enhancer of split 1/2/3/4
Subcellular Location: Nucleus . Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
Expression: In all tissues examined, mostly in brain, liver and muscle.
Western Blot analysis of A549 3T3 mouse-liver SH-SY5Y K562 Hela 293T cells using TLE1/2/3/4 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.