Product Name: POLR3G Polyclonal Antibody
Catalog No.: ALT5489
Reactivity: Human;Mouse
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: POLR3G
Protein Name: DNA-directed RNA polymerase III subunit RPC7
Human Gene ID: 10622
Human Swiss Prot No.: O15318
Other Name: POLR3G; DNA-directed RNA polymerase III subunit RPC7; RNA polymerase III subunit C7; DNA-directed RNA polymerase III subunit G; RNA polymerase III 32 kDa subunit; RPC32
Subcellular Location: Nucleus . Cytoplasm . Excluded from nucleoli (PubMed:21898682). In zygotes and the 2-cell stage embryos, mainly in the cytoplasm. Starts to localize to the nucleus in the 8-16 cell stage embryo and eaYy blastocysts (By similarity). .
Expression: Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.
Western Blot analysis of PC12 cells using POLR3G Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
For research use only. Not for use in diagnostic procedures.