Product Name: Ki-67 Polyclonal Antibody
Catalog No.: ALT2467
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MKI67
Protein Name: Antigen KI-67
Human Gene ID: 4288
Human Swiss Prot No.: P46013
Other Name: MKI67; Antigen KI-67
Subcellular Location: Chromosome . Nucleus . Nucleus, nucleolus . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). .
Expression: Epithelium,
Immunofluorescence analysis of COS7 cells, using Ki67 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67 Antibody. The picture on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.