Product Name: EDG-1 Polyclonal Antibody
Catalog No.: ALT1460
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: S1PR1
Protein Name: Sphingosine 1-phosphate receptor 1
Human Gene ID: 1901
Human Swiss Prot No.: P21453
Other Name: S1PR1; CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD antigen CD363
Subcellular Location: Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.
Expression: Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Immunofluorescence analysis of COS7 cells, using EDG1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO205 cells, using EDG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using EDG1 antibody.
For research use only. Not for use in diagnostic procedures.