Product Name: Rad17 (phospho Ser646) Polyclonal Antibody
Catalog No.: ALP1203
Reactivity: Human;Mouse;Rat
Applications: WB; ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name: RAD17
Protein Name: Cell cycle checkpoint protein RAD17
Human Gene ID: 5884
Human Swiss Prot No.: O75943
Other Name: RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog
Subcellular Location: Nucleus . Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Expression: Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Western Blot analysis of extracts from K562 cells, using Phospho-Rad17 (S646) Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.