Product Name: ATF-2 (phospho Ser480) Polyclonal Antibody
Catalog No.: ALP0909
Reactivity: Human;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ATF2
Protein Name: Cyclic AMP-dependent transcription factor ATF-2
Human Gene ID: 1386
Human Swiss Prot No.: P15336
Other Name: ATF2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding pro
Subcellular Location: Nucleus. Cytoplasm. Mitochondrion outer membrane. Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
Expression: Ubiquitously expressed, with more abundant expression in the brain.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ATF2 (Phospho-Ser480) Antibody
Immunofluorescence analysis of HeLa cells, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with Anisomycin 25ug/ml 30′, using ATF2 (Phospho-Ser480) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.