Product Name: JNK1/2/3 (phospho Tyr185) Polyclonal Antibody
Catalog No.: ALP0843
Reactivity: Human;Mouse;Rat
Applications: IF/ICC;WB;IHC-p;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: IF: 1:50-200 Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MAPK8/9/10
Protein Name: Mitogen-activated protein kinase 8/9/10
Human Gene ID: 5599/5601/5602
Human Swiss Prot No.: P45983/P45984/P53779
Other Name: MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mi
Subcellular Location: Cytoplasm . Nucleus . Cell junction, synapse . In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus. .
Expression: Brain,Epithelium,Fetal brain,Lung,Pooled,Testis,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SAPK/JNK (Phospho-Tyr185) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using SAPK/JNK (Phospho-Tyr185) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using SAPK/JNK (Phospho-Tyr185) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.