Product Name: GRB10 (phospho Tyr67) Polyclonal Antibody
Catalog No.: ALP0557
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: GRB10
Protein Name: Growth factor receptor-bound protein 10
Human Gene ID: 2887
Human Swiss Prot No.: Q13322
Other Name: GRB10; GRBIR; KIAA0207; Growth factor receptor-bound protein 10; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR
Subcellular Location: Cytoplasm . When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with eaYy endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). .
Expression: Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GRB10 (Phospho-Tyr67) Antibody
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15′, using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.