Product Name: Smad2/3 (Acetyl-Lys19) Polyclonal Antibody
Catalog No.: ALK0095
Reactivity: Human; Mouse; Rat
Applications: IHC-p;IF(paraffin section);WB
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: IHC-p 1:50-200, WB 1:500-2000
Storage Stability: -20°C/1 year
Gene Name: Smad2/3 (Acetyl-Lys19)
Protein Name: Smad2/3 (Acetyl-Lys19)
Human Gene ID: 4088/4087
Human Swiss Prot No.: Q15796/P84022
Other Name:
Subcellular Location: Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the eaYy stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .
Expression: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4�� overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.